The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’)

By M. Bairu, C. W. Fennell, J. van Staden. 2006

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Somaclonal Variation in Tissue Culture: A Case Study with Olive (2012, background): “The frequency of somaclonal variation increases as the number of subcultures and their duration increases, especially in cell suspensions and callus cultures [56, 57, 58].”
In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis (2010, background): “However, this enhances the probability of cell mutations and the genetic instability of regenerated plants ( Bairu et al. 2006 ), in a process known as somaclonal variation (Larkin and Scowcroft 1981), and makes assessment of the genetic fidelity of the regenerants necessary, by e.g., the use of mol”
Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System (2017, background): “…procedures, clonal integrity is required, so callus is not used and conservative use of plant growth regulators is maintained to reduce the possibility of somaclonal variation (Nehra et al. 1992; Skirvin et al. 1994; Sahijram et al. 2003; Bairu et al. 2006; Bairu et al. 2008; Bairu et al. 2011).”
Somaclonal variation in plants: causes and detection methods (2011, background): “Number and duration of subcultures Increasing the number of subculture and their duration enhances the rate of somaclonal variations, especially cell suspension and callus cultures (Reuveni and Israeli 1990; Rodrigues et al. 1998; Bairu et al. 2006).”