Optetrode: a multichannel readout for optogenetic control in freely moving mice

By Polina Anikeeva, A. Andalman, Ilana Witten, Melissa R. Warden, I. Goshen, Logan Grosenick, Lisa A. Gunaydin, Loren M. Frank, Karl Deisseroth. Nature Neuroscience, 2011

382 citations recorded by the source corpus.

Recent advances in optogenetics have improved the precision with which defined circuit elements can be controlled optically in freely moving mammals; in particular, recombinase-dependent opsin viruses, used with a growing pool of transgenic mice expressing recombinases, allow manipulation of specific cell types. However, although optogenetic control has allowed neural circuits to be manipulated in increasingly powerful ways, combining optogenetic stimulation with simultaneous multichannel electrophysiological readout of isolated units in freely moving mice remains a challenge. We designed and validated the optetrode, a device that allows for colocalized multi-tetrode electrophysiological recording and optical stimulation in freely moving mice. Optetrode manufacture employs a unique optical fiber-centric coaxial design approach that yields a lightweight (2 g), compact and robust device that is suitable for behaving mice. This low-cost device is easy to construct (2.5 h to build without specialized equipment). We found that the drive design produced stable high-quality recordings and continued to do so for at least 6 weeks following implantation. We validated the optetrode by quantifying, for the first time, the response of cells in the medial prefrontal cortex to local optical excitation and inhibition, probing multiple different genetically defined classes of cells in the mouse during open field exploration.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/607df9081d1232b143a7ab0b5ca766063ae8b205

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Citations in the corpus

Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals. (2014, methodology): “However, construction and use of silicon probe devices with etched optical fibers that allow precise local delivery of light energy are currently done manually, and only few laboratories have the capability to use such methods routinely (Anikeeva et al. 2012; Royer et al. 2010, 2012; Stark et al. 20”
Soft materials in neuroengineering for hard problems in neuroscience. (2015, background): “These hybrid probes, sometimes known as optrodes, represent powerful platforms for experimental neuroscience (Anikeeva et al., 2012; Wu et al., 2013b; Ozden et al., 2013).”