The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

By J. Huggett, C. Foy, Vladimir Benes, K. Emslie, J. Garson, R. Haynes, Jan Hellemans, M. Kubista, Reinhold D. Mueller, T. Nolan and 5 more. Clinical Chemistry, 2013

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The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. (2020, positive): “Since the publication of the guidelines for the Minimum Information for Publication of Quantitative Digital PCR Experiments (dMIQE) (1), digital PCR (dPCR) has seen considerable technological development with a plethora of new applications.”
dPCR: A Technology Review (2018, methodology): “Proper assay design and validation are critical to minimize typical issues arising from molecular dropout, false positives, or poor signal thresholding [13,44].”
An overview of microRNAs: Biology, functions, therapeutics, and analysis methods (2018, background): “Target molecules copies in the reaction are estimated under the assumption of a Poisson’s distribution (Huggett et al., 2013).”
Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease (2019, background): “, 2011), but the technique requires optimization, proper standard curves, and ideally the inclusion of spiked-in exogenous miRNAs (Huggett et al., 2013; Mitchell et al., 2008; Zhuang et al., 2012a).”