Magnetic techniques for the isolation and purification of proteins and peptides
By I. Šafařík, M. Šafaříková. BioMagnetic Research and Technology, 2004
494 citations recorded by the source corpus.
Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.
Subfields: Materials Chemistry, Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/6052d97b8bcaa56116c240997b8404fa18d67091
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In Materials Chemistry
Citations in the corpus
- Magnetic nano- and microparticles in biotechnology (2009, unknown): “Magnetic separation of proteins has been summarized in recent review papers (Franzreb et al., 2006; Safarik & Safarikova, 2004).”
- Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. (2007, unknown): “In this study, we provide the first report that Fe3O4 MNPs possess intrinsic peroxidase-like activity comparable to that of an enzymecatalysed reaction by demonstrating that (1) Fe3O4 MNPs catalysed the reaction of different peroxidase substrates such as TMB, DAB and OPD to give the same colour chan”
- Magnetic separations: From steel plants to biotechnology (2009, background): “Immobilization of ligands on the magnetic adsorbents for the capture of the target species is crucial; this is perhaps one reason that conventional liquid column chromatography remains the gold standard for demanding purification processes (Safarik and Safarikova, 2004).”