Profiling Membrane Lipids in Plant Stress Responses

By Ruth Welti, Weiqi Li, Maoyin Li, Y. Sang, Homigol Biesiada, Hanyang Zhou, C. Rajashekar, T. Williams, Xuemin Wang. Journal of Biological Chemistry, 2002

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A sensitive approach based on electrospray ionization tandem mass spectrometry has been employed to profile membrane lipid molecular species in Arabidopsis undergoing cold and freezing stresses. Freezing at a sublethal temperature induced a decline in many molecular species of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) but induced an increase in phosphatidic acid (PA) and lysophospholipids. To probe the metabolic steps generating these changes, lipids ofArabidopsis deficient in the most abundant phospholipase D, PLDα, were analyzed. The PC content dropped only half as much, and PA levels rose only half as high in the PLDα-deficient plants as in wild-type plants. In contrast, neither PE nor PG levels decreased significantly more in wild-type plants than in PLDα-deficient plants. These data suggest that PC, rather than PE and PG, is the majorin vivo substrate of PLDα. The action of PLDα during freezing is of special interest because Arabidopsis plants that are deficient in PLDα have improved tolerance to freezing. The greater loss of PC and increase in PA in wild-type plants as compared with PLDα-deficient plants may be responsible for destabilizing membrane bilayer structure, resulting in a greater propensity toward membrane fusion and cell death in wild-type plants.

Subfields: Biochemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/604ad4a413b190268d17ae1b6a1d6e06cb9ab518

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Citations in the corpus

Phospholipids as Plant Growth Regulators (2006, background): “Lysophospholipids are present in membranes only in trace amounts but their concentration increases during acclimation to freezing (Welti et al. 2002), cell expansion (Scherer 2002) and in response to wounding (Lee et al.”
Cold stress tolerance mechanisms in plants. A review (2010, background): “In addition to the well-established harmful effect of cold stress alterations in lipid composition of the biomembranes, affecting their fluidity (Senser and Beck, 1982; Quinn, 1985; Williams, 1990; Welti et al., 2002), certain additional factors may also contribute to damage induced by cold stress.”
Biology and Biochemistry of Plant Phospholipases (2011, background): “PLDα1-deficient Arabidopsis exhibited enhanced freezing tolerance, possibly through reduced disruption of the polar lipid composition of the membrane, resulting in greater membrane stability under freezing conditions (Welti et al., 2002).”
Exogenously-supplied trehalose protects thylakoid membranes of winter wheat from heat-induced damage (2010, background): “…of the protein and interact predominantly with hydrophobic amino acids that are in close contact with the protein’s cofactors (Camara-Artigas et al. 2002), and increased PC content in trehalose-pretreatment might promote bilayer phase formation and maintain membrane competency (Welti et al. 2002).”
The Arabidopsis Cold-Responsive Transcriptome and Its Regulation by ICE1w⃞ (2005, background): “Studies with Arabidopsismutant or overexpression lines of PLDs supported their involvement in plant cold tolerance (Welti et al., 2002; Li et al., 2004).”
Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance (2008, background): “As reported earlier, these changes are resulting from the lipid hydrolysis via the activation of hydrolytic enzymes (Welti et al. 2002).”
UvA-DARE ( Digital Academic Repository ) Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase (2017, background): “PtdOH levels in plants are approximately 2 mol% of total phospholipids (Welti et al., 2002) which likely represents ER- and plastid-localized PtdOH as precursor and turnover product of structural glycerolipids.”
Profiling lipid changes in plant response to low temperatures (2006, methodology): “Thus, suppression of PLDa1 may decrease the propensity of membrane lipids to undergo a transition from lamellar to hexagonal II phase, thus increasing freezing tolerance (Welti et al. 2002).”