Enzymatic assembly of DNA molecules up to several hundred kilobases

By D. G. Gibson, L. Young, R. Chuang, J. C. Venter, Clyde A. Hutchison, O. Hamilton, Smith. 2009

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In Molecular Biology

Citations in the corpus

Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2 (2020, positive): “The RBD-16GS-I53-50A fusion was cloned into pCMV/R using the Xba1 and AvrII restriction sites and Gibson assembly (Gibson et al., 2009).”
In vitro reconstitution of Wnt acylation reveals structural determinants of substrate recognition by the acyltransferase human Porcupine (2018, methodology): “For a fluorescence protease protection (FPP) assay, an eGFP coding sequence was inserted at the N terminus, C terminus, or interhelical loops of human PORCN by exponential megapriming PCR (38) or Gibson assembly (39) cloning methods.”
Enzymatic Assembly of Overlapping DNA Fragments (2011, methodology): “A 50 C ISO assembly system has been optimized using the activities of the 50-T5 exonuclease (T5 exo), PhusionÒ DNA polymerase (PhusionÒ pol), and Taq lig (Gibson et al., 2009; Fig.”
Multiplex Assessment of Protein Variant Abundance by Massively Parallel Sequencing (2018, methodology): “All non-library related plasmid modifications were performed with Gibson assembly 57 .”
BglBricks: A flexible standard for biological part assembly (2010, methodology): “More recently, there have been reports of a single-reaction DNA shuffling method based on type IIs restriction enzymes, known as Golden Gate Shuffling [28], as well as a single-reaction isothermal method for the assembly of multiple overlapping DNA molecules [29].”
Acetyl-CoA Induces Cell Growth and Proliferation by Promoting the Acetylation of Histones at Growth Genes (2011, methodology): “Fusion PCR or isothermal assembly (Gibson et al., 2009) was then performed to fuse the fragments together and the product was used for transformation.”
Complex cellular logic computation using ribocomputing devices (2017, methodology): “The resulting DNAs were then inserted into plasmid backbones using 30-bp homology domains via Gibson assembly 30 .”
Learning the sequence determinants of alternative splicing from millions of random sequences. (2015, neutral): “(Gibson et al., 2009) using degenerate oligonucleotides (IDTDNA).”
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system (2013, methodology): “Plasmid pdCas9 was constructed by introducing D10A and H840A mutations to cas9 on pCas9 through amplification of this plasmid with primers B337/B340 and B338/ B339, followed by Gibson assembly (34) of the two products.”