In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu
By Michiko Takahashi, Tomoko Nozoye, N. Kitajima, Naoki Fukuda, A. Hokura, Y. Terada, I. Nakai, Yasuhiro Ishimaru, Takanori Kobayashi, H. Nakanishi and 1 more. Plant and Soil, 2009
116 citations recorded by the source corpus.
To investigate the flow of the metal nutrients iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) during rice seed germination, we performed microarray analysis to examine the expression of genes involved in metal transport. Many kinds of metal transporter genes were strongly expressed and their expression levels changed during rice seed germination. We found that metal transporter genes such as ZIP family has tendency to decrease in their expressions during seed germination. Furthermore, imaging of the distribution of elements (Fe, Mn, Zn, and Cu) was carried out using Synchrotron-based X-ray microfluorescence at the Super Photon ring-8 GeV (SPring-8) facility. The change in the distribution of each element in the seeds following germination was observed by in vivo monitoring. Iron, Mn, Zn, and Cu accumulated in the endosperm and embryos of rice seeds, and their distribution changed during rice seed germination. The change in the patterns of mineral localization during germination was different among the elements observed.
Subfields: Plant Science, Pollution
View Paper: https://www.semanticscholar.org/paper/5ea3e084be27c0c3b37c95d3c60ee92b7ab3bc59
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Plant Science
Citations in the corpus
- Zn Uptake and Translocation in Rice Plants (2011, background): “NA, DMA, and Zn is mobilized during germination in rice (Takahashi et al. 2009).”
- Evaluating rice germplasm for iron and zinc concentration in brown rice and seed dimensions (2012, methodology): “[13] revealed that Zn is most abundant in the embryo and in the aleurone layer using X-ray fluorescence imaging.”
- Iron Uptake and Loading into Rice Grains (2010, methodology): “the rice embryo, Fe localizes not only to the epithelium, scutellum, and coleoptile, but also to the leaf primordium and radicle (Takahashi et al. 2009).”