Systems biotechnology for strain improvement.

By Sang Yup Lee, Dong-Yup Lee, Tae Yong Kim. Trends in Biotechnology, 2005

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Fermentative butanol production by clostridia (2008, methodology): “The target genes to be engineered to achieve these goals can be identified by genome-scale metabolic flux analysis and omics studies (Lee et al., 2005).”
Constraint-based models predict metabolic and associated cellular functions (2014, methodology): “In the past ten years, many algorithms have been developed for predicting useful genetic manipulation strategies for metabolic engineering 67,68 .”