Population Genetics and Phylogenetics of the Endangered American Burying Beetle, Nicrophorus americanus (Coleoptera: Silphidae)
By Allen L. Szalanski, D. Sikes, Richard Bischof, M. Fritz. 2000
93 citations recorded by the source corpus.
Abstract The burying beetle Nicrophorus americanus Olivier is an endangered species known to occur in disjunct populations in 6 states. Parsimony and maximum likelihood analysis of the nuclear ribosomal DNA first internal transcribed spacer (ITS1) sequences from 10 Nicrophorinae species revealed N. americanus to form a distinct clade with N. orbicollis Say. Genetic variation within and among 5 N. americanus populations, collected from South Dakota, Nebraska, Oklahoma, Arkansas, and Rhode Island, was studied. Ribosomal DNA ITS1 sequences from 14 beetles revealed 48 polymorphic and 20 informative nucleotide sites. N. americanus genetic divergence was between 0.16 and 4.76%. We found little evidence that these 5 populations have maintained unique genetic variation. No nucleotide sites were found that were diagnostic for any of the 5 populations examined, indicating that these populations may not be necessarily treated as separate, independent objects of conservation. However, further genetic investigation is warranted before translocations are attempted among the remaining populations of the American burying beetle.
Subfields: Ecology, Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/5def3bd1ddab79f8368897a83826f4b61fb002cd
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- J. Austin
- R. Scheffrahn
- Allen L. Szalanski
- M. Messenger
- A. Bagnères
- S. Dronnet
- P. Uva
- A. Kence
Citations in the corpus
- A Comparative Genetic Analysis of the Subterranean Termite Genus Reticulitermes (Isoptera: Rhinotermitidae) (2002, methodology): “PCR reactions were conducted using 1 l of the extracted DNA (Szalanski et al. 2000), with a proÞle consisting of 35 cycles of 94 C for 45 s, 46 C for 45 s, and 72 C for 60 s. AmpliÞed DNA from individual termites was puriÞed and concentrated using Microcon-PCR Filter Units (Millipore, Bedford, MA).”
- Genetic Evidence for the Synonymy of Two Reticulitermes Species: Reticulitermes flavipes and Reticulitermes santonensis (2005, methodology): “The PCR reactions were conducted with 1 l of the extracted DNA (Szalanski et al. 2000), having a thermocycler proÞle consisting of 35 cycles of 94 C for 45 s, 46 C for 45 s, and 72 C for 60 s. AmpliÞed DNA from individual termites was puriÞed and concentrated with minicolumns according to the…”