MicroRNAs as master regulators of the plant NB-LRR defense gene family via the production of phased, trans-acting siRNAs.
By J. Zhai, D. Jeong, E. De Paoli, Sunhee Park, B. Rosen, Yupeng Li, Alvaro J. González, Zhe Yan, S. Kitto, M. Grusak and 6 more. Genes & Development, 2011
674 citations recorded by the source corpus.
Legumes and many nonleguminous plants enter symbiotic interactions with microbes, and it is poorly understood how host plants respond to promote beneficial, symbiotic microbial interactions while suppressing those that are deleterious or pathogenic. Trans-acting siRNAs (tasiRNAs) negatively regulate target transcripts and are characterized by siRNAs spaced in 21-nucleotide (nt) "phased" intervals, a pattern formed by DICER-LIKE 4 (DCL4) processing. A search for phased siRNAs (phasiRNAs) found at least 114 Medicago loci, the majority of which were defense-related NB-LRR-encoding genes. We identified three highly abundant 22-nt microRNA (miRNA) families that target conserved domains in these NB-LRRs and trigger the production of trans-acting siRNAs. High levels of small RNAs were matched to >60% of all ∼540 encoded Medicago NB-LRRs; in the potato, a model for mycorrhizal interactions, phasiRNAs were also produced from NB-LRRs. DCL2 and SGS3 transcripts were also cleaved by these 22-nt miRNAs, generating phasiRNAs, suggesting synchronization between silencing and pathogen defense pathways. In addition, a new example of apparent "two-hit" phasiRNA processing was identified. Our data reveal complex tasiRNA-based regulation of NB-LRRs that potentially evolved to facilitate symbiotic interactions and demonstrate miRNAs as master regulators of a large gene family via the targeting of highly conserved, protein-coding motifs, a new paradigm for miRNA function.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/5d585cae66d551001eaa30d21a2dc463d54b7504
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Citations in the corpus
- A MicroRNA Superfamily Regulates Nucleotide Binding Site–Leucine-Rich Repeats and Other mRNAs[W][OA] (2012, methodology): “NBS-LRR Targets of Medicago miR2118 Family Members and slmiR482 in Arabidopsis.”
- The Amborella Genome and the Evolution of Flowering Plants (2013, background): “Similar totheresultsfor Medicago ( 72 ) and members of Solanaceae ( 73 , 74 ), several phased siRNA loci were nucleotide binding site – leucine-rich repeat (NB-LRR) disease resistance genes targeted by miRNAs in the miR482/2118 superfamily.”