A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project
By Latarsha J. Carithers, Kristin G. Ardlie, M. Barcus, P. Branton, Angela Britton, Stephen A. Buia, C. Compton, D. DeLuca, Joanne Peter-Demchok, Ellen T. Gelfand and 17 more. Biopreservation and Biobanking, 2015
785 citations recorded by the source corpus.
The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.
Subfields: Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/5ca3c0290848127ac4b33e39d90d651cea79d2c7
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In Genetics
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Citations in the corpus
- Benchmark and integration of resources for the estimation of human transcription factor activities (2018, methodology): “We used GTEx v6 human gene expression data (Carithers et al. 2015) from control donors to infer transcriptional regulatory networks for healthy tissues.”
- Anchor regression: Heterogeneous data meet causality (2018, methodology): “iction problem under distributional shifts. The code is available on github.com/rothenhaeusler. 5.1 Genotype-tissue expression The data was obtained from the Genotype-Tissue Expression (GTEx) portal [Carithers et al., 2015]. One of the GTEx datasets contains gene expression data from 53 tissues of 7”
- Massively-parallel single nucleus RNA-seq with DroNc-seq (2017, neutral): “Samples were originally collected from recently deceased, non-diseased donors 18,23 .”
- The Genotype-Tissue Expression (GTEx) Project (2015, background): “National Cancer Institute (NCI) and its partners in collecting high quality post-mortem biospecimens for a major National Institutes of Health (NIH) research program, the Genotype-Tissue Expression (GTEx) project.(1) GTEx aims to evaluate the relationship between genetic variation and gene expressio”