Computational Design of an Unnatural Amino Acid Dependent Metalloprotein with Atomic Level Accuracy

By J. Mills, Sagar D. Khare, Jill Bolduc, F. Forouhar, Vikram Khipple Mulligan, S. Lew, J. Seetharaman, L. Tong, Barry L. Stoddard, David Baker. 2013

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Genetically encoded unnatural amino acids could facilitate the design of proteins and enzymes of novel function, but correctly specifying sites of incorporation, and the identities and orientations of surrounding residues represents a formidable challenge. Computational design methods have been used to identify optimal locations for functional sites in proteins and design the surrounding residues, but have not incorporated unnatural amino acids in this process. We extended the Rosetta design methodology to design metalloproteins in which the amino acid (2,2’-bipyridin-5yl)alanine (Bpy-Ala) is a primary ligand of a bound metal ion. Following initial results that indicated the importance of buttressing the Bpy-Ala amino acid, we designed a buried metal binding site with octahedral coordination geometry consisting of Bpy-Ala, two protein based metal ligands, and two metal bound water molecules. Experimental characterization revealed a Bpy-Ala mediated metalloprotein with the ability to bind divalent cations including Co2+, Zn2+, Fe2+, and Ni2+, with a Kd for Zn2+ of ~40 pM. X-ray crystallographic analysis of the designed protein shows only slight deviation from the computationally designed model.

Subfields: Electrical and Electronic Engineering, Molecular Biology

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