Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases δ-selinene synthase and γ-humulene synthase

By D. Little, R. Croteau. 2002

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Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defence of conifers against insects and pathogens. (2006, methodology): “Two sesquiterpene synthases from grand fir, δ-selinene synthase and γ-humulene synthase, have also been examined using site-directed mutagenesis and truncated constructs (Little & Croteau, 2002).”
Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants. (2009, methodology): “#, d-elemene could have been formed from germacrene C in the GC injector at the 250 C temperature used; , the purified enzyme produced germacrene B (33%) as main product (Little and Croteau, 2002); nd, the enzymes were not expressed and characterized; ^, identical to AAF80333.”