The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development.

By Sumio Isogai, Masaharu Horiguchi, B. Weinstein. Developmental Biology, 2001

903 citations recorded by the source corpus.

We have used confocal microangiography to examine and describe the vascular anatomy of the developing zebrafish, Danio rerio. This method and the profound optical clarity of zebrafish embryos make it possible to view the entire developing vasculature with unprecedented resolution. A staged series of three-dimensional images of the vascular system were collected beginning shortly after the onset of circulation at 1 day postfertilization through early- to midlarval stages at approximately 7 days postfertilization. Blood vessels in every region of the animal were imaged at each stage, and detailed "wiring patterns" were derived describing the interconnections between every major vessel. We present an overview of these data here in this paper and in an accompanying Web site "The interactive atlas of zebrafish vascular anatomy" online at (http://eclipse.nichd.nih.gov/nichd/lmg/redirect.html). We find a highly dynamic but also highly stereotypic pattern of vascular connections, with different sets of primitive embryonic vessels severing connections and rewiring in new configurations according to a reproducible plan. We also find that despite variation in the details of the vascular anatomy, the basic vascular plan of the developing zebrafish shows strong similarity to that of other vertebrates. This atlas will provide an invaluable foundation for future genetic and experimental studies of vascular development in the zebrafish.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/59e93cb024a2c570da5be958ceb5949c87bab3df

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Citations in the corpus

Cellular and molecular analyses of vascular tube and lumen formation in zebrafish (2005, background): “As Vegfc can also interact with Vegfr2 (Joukov et al., 1996; Ober et al., 2004), and the major receptor for Vegfc, Vegfr3/Flt4, is not Development 132 (23) Research article D ev el op m en t 5207Vascular tube formation in zebrafish expressed in the pharyngeal endoderm, it is plausible that the phary”
In vivo imaging of embryonic vascular development using transgenic zebrafish. (2002, methodology): “For example, we have injected fluorescent microspheres into the circulation of zebrafish embryos fol- lowed by confocal laser microscopy to characterize wild type or mutant circulatory networks at various time points (Isogai et al., 2001; Weinstein et al., 1995).”
Identification of RNF213 as a Susceptibility Gene for Moyamoya Disease and Its Possible Role in Vascular Development (2011, background): “5 dpf, despite massive development of smaller-caliber vessels in the head through to 7 dpf [35].”
Consensus guidelines for the use and interpretation of angiogenesis assays (2018, background): “Up to approximately 7 dpf, the pattern of blood vessels observed should be compared to the published staged atlas of vascular anatomy [328].”