The Existence of a Second Vesicular Glutamate Transporter Specifies Subpopulations of Glutamatergic Neurons
By E. Herzog, G. Bellenchi, Christelle Gras, V. Bernard, P. Ravassard, Cecile Bedet, B. Gasnier, B. Giros, S. El Mestikawy. Journal of Neuroscience, 2001
624 citations recorded by the source corpus.
Before their exocytotic release during stimulation of nerve terminals, nonpeptide neurotransmitters are loaded into synaptic vesicles by specific transporters. Recently, a protein initially identified as brain-specific Na(+)-dependent inorganic phosphate transporter I (BNPI) has been shown to represent a vesicular glutamate transporter (VGLUT1). In this study, we investigated whether a highly homologous "differentiation-associated Na(+)-dependent inorganic phosphate transporter" (DNPI) is involved in glutamatergic transmission. Vesicles isolated from BON cells expressing recombinant DNPI accumulated l-glutamate with bioenergetical and pharmacological characteristics identical to those displayed by VGLUT1 and by brain synaptic vesicles. Moreover, DNPI localized to synaptic vesicles, at synapses exhibiting classical excitatory features. DNPI thus represents a novel vesicular glutamate transporter (VGLUT2). The distributions of each VGLUT transcript in brain were highly complementary, with only a partial regional and cellular overlap. At the protein level, we could only detect either VGLUT1- or VGLUT2-expressing presynaptic boutons. The existence of two VGLUTs thus defines distinct subsets of glutamatergic neurons.
Subfields: Biochemistry, Cell Biology, Cellular and Molecular Neuroscience
View Paper: https://www.semanticscholar.org/paper/59de054456db80467dc7bb7be1824837b2c2364d
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cell Biology
In Cellular and Molecular Neuroscience
Citations in the corpus
- LETTER TO NEUROSCIENCE DIFFERENTIAL DISTRIBUTION OF VESICULAR GLUTAMATE TRANSPORTERS IN THE RAT CEREBELLAR CORTEX (2003, background): “VGluT1 immunoreactivity (Bellocchio et al., 1998) and VGluT2 immunoreactivity (Fremeau et al., 2001; Fujiyama et al., 2001; Herzog et al., 2001; Kaneko and Fujiyama, 2002) were electron-microscopically located on synaptic vesicles of the axon terminals which made asymmetric, putatively excitatory…”
- VGLUTs define subsets of excitatory neurons and suggest novel roles for glutamate. (2004, background): “34 Herzog, E. et al. (2001) The existence of a second vesicular glutamate”
- A Third Vesicular Glutamate Transporter Expressed by Cholinergic and Serotoninergic Neurons (2002, methodology): “BON cells stably transfected with rat VGLUT2 or vesicular inhibitory amino acid transporter (VIAAT) cDNAs were used as controls (Herzog et al., 2001).”
- Cortico-Basal Ganglia Reward Network: Microcircuitry (2009, neutral): “The detailed connectivity of these cells will take time to decipher, given their low numbers and the fact that they can only be detected by in situ hybridization for VGlut2 mRNA, a selective marker of subcortical glutamate neurons (Herzog et al, 2001).”
- Presynaptic Control of Striatal Glutamatergic Neurotransmission by Adenosine A1–A2A Receptor Heteromers (2006, background): “detected in single glutamatergic nerve terminals, identified by immunostaining using antibodies against vesicular glutamate transporters type 1 and 2 (vGluT1 and vGluT2), which are expressed in glutamatergic neurons (Fremeau et al., 2001; Herzog et al., 2001) (Fig.”