Methods in Cellular Imaging

By A. Periasamy. Methods in Physiology, 2001

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Fluorescence lifetime imaging – techniques and applications (2012, methodology): “Because of its dependence on the distance on the molecular scale FRET has become an important tool of cell biology (Patterson et al., 2000; Periasamy, 2001; Hink et al., 2003; Periasamy & Clegg, 2009).”
SINGLE-PARTICLE TRACKING (2009, background): “The imaging quality of a microscope is specified by the point spread function [5–9], which describes how a point source of light in the object plane is broadened by diffraction to a spot in the image plane, an ellipsoid in 3D.”
Imaging protein molecules using FRET and FLIM microscopy. (2005, background): “However, the desire to capture microsecond or nanosecond cellular changes and interactions in living cells in their natural environment has led to the development of increasingly sophisticated imaging technologies [1–5].”