Parallel tagged sequencing on the 454 platform

By M. Meyer, U. Stenzel, M. Hofreiter. Nature Protocols, 2008

336 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/57289b5cc207ff23a345550f9b50deaee0d514f0

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In Molecular Biology

Citations in the corpus

High‐throughput DNA sequencing – concepts and limitations (2010, background): “region/channel, and later separated (computationally) based on their barcode sequence [70–73].”
Applications of next-generation sequencing to phylogeography and phylogenetics. (2013, methodology): “( 20 10 ) C an le ve ra ge da ta fr om ex pr es si on st u di es Sk ew ed re ad di st ri bu ti on s ca n ou ts tr ip co ve ra ge ,m ak in g it di ffi cu lt to fi n d or th ol og ou s lo ci Le ve ra gi n g ex is ti n g cD N A li br ar ie s et al., 2007) or ligation (Meyer et al., 2008).”
Ultraconserved elements anchor thousands of genetic markers spanning multiple evolutionary timescales. (2012, methodology): “Multiplex PCR and methods for tagging and pooling PCR amplicons address the output of MPS platforms (Meyer et al. 2008), but these methods do not scale at the input stage, that is, they do not remove the onerous step of amplifying tens or hundreds of orthologous loci in multiple taxonomically distan”
Ecological genetics of invasive alien species (2011, methodology): “For example, MG-RAST is a fully-automated service for annotating metagenome samples including phylogenetic classification (Meyer et al. 2008).”
Single‐tube library preparation for degraded DNA (2017, background): “In previous protocols, a high concentration of T4 DNA Polymerase (0.1-0.5 U/L) has been common (Margulies et al. 2005; Kucera & Nichols, 2008; Meyer et al. 2008; Meyer & Kircher, 2010; Briggs & Heyn, 2012).”
Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels (2012, background): “[3] conformed to their expected minimum Hamming distance.”
Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform (2011, neutral): “The comparatively high contamination estimate for the SP-CAP experiment is also supported by another—albeit less powerful—analysis, which can be performed by counting the number of sequences derived from unused indexes (24) (see Supplementary Methods section).”