Structures of the human and Drosophila 80S ribosome

By A. Anger, Jean-Paul Armache, O. Berninghausen, M. Habeck, Marion Subklewe, Daniel N. Wilson, Roland Beckmann. Nature, 2013

597 citations recorded by the source corpus.

Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/56d937483db5523b422295116eb15699b5a7e227

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In Molecular Biology

Citations in the corpus

A census of human RNA-binding proteins (2014, background): “Eukaryotic ribosomes have 80 core ribosomal proteins (Anger et al., 2013), the large ribosomal subunit contains 47 proteins, while the small subunit”
Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB (2013, background): “Moreover, down-sampling can have a deleterious effect on the level of detail that can be visualised.”
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA (2015, background): “Human ribosomes contain several long, G:C rich, base-paired RNA expansion segments forming large tentacle-like hairpin structures of substantial double-stranded nature (Anger et al., 2013).”
Recent advances in RNA structurome (2022, background): “At a global level, the structures and interactions of RNAs in RNP machines, such as ribosome and spliceosome, undergo dynamic changes during their assembly and function to ensure faithful and highly efficient cellular progresses in animal cells (Anger et al., 2013; Wilkinson et al., 2020).”
The Jigsaw Puzzle of mRNA Translation Initiation in Eukaryotes: A Decade of Structures Unraveling the Mechanics of the Process (2018, background): “These ESs can be of substantial size, up to several hundreds of nucleotides, and can be accompanied by extensions of ribosomal proteins, as demonstrated by the pioneering 40S, 60S, and 80S structures obtained by X-ray crystallography and cryoEM, to which we owe the exquisite and detailed structural ”