Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy
By N. Olivier, Miguel Angel Luengo-Oroz, L. Duloquin, E. Faure, T. Savy, I. Veilleux, X. Solinas, D. Débarre, Paul Bourgine, Andrés Santos and 2 more. Science, 2010
359 citations recorded by the source corpus.
Zebrafish Development in 3D Vertebrate development has classically been characterized qualitatively, but—by combining expertise in physics, mathematics, and biology—Olivier et al. (p. 967) used label-free conformal nonlinear time-lapse microscopy and image analysis to calculate the spatiotemporal cell lineage of zebrafish embryos throughout their first 10 division cycles. The work reconstructs complete lineage trees, annotated with cell-shape measurements, and allows for visualization with interactive tools. Time-lapse recording characterizes the rhythm and cleavage pattern of the embryo during early stages of development. Quantifying cell behaviors in animal early embryogenesis remains a challenging issue requiring in toto imaging and automated image analysis. We designed a framework for imaging and reconstructing unstained whole zebrafish embryos for their first 10 cell division cycles and report measurements along the cell lineage with micrometer spatial resolution and minute temporal accuracy. Point-scanning multiphoton excitation optimized to preferentially probe the innermost regions of the embryo provided intrinsic signals highlighting all mitotic spindles and cell boundaries. Automated image analysis revealed the phenomenology of cell proliferation. Blastomeres continuously drift out of synchrony. After the 32-cell stage, the cell cycle lengthens according to cell radial position, leading to apparent division waves. Progressive amplification of this process is the rule, contrasting with classical descriptions of abrupt changes in the system dynamics.
Subfields: Biophysics, Structural Biology
View Paper: https://www.semanticscholar.org/paper/5693909e35ea088000380eaf4f49b5aebb0e2f21
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In Biophysics
Citations in the corpus
- Forces in tissue morphogenesis and patterning. (2013, positive): “Finally, althoughmany studies have been performed on two-dimensional epithelial cell sheets, an important challenge will be to further extend recent segmentation methods, cinematic measurements, and mechanical models to three dimensions (Olivier et al., 2010; Kennaway et al., 2011; Gelbart et al., 2”
- Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data (2014, background): “For a particularly cost-efficient build, slower CPUs and hard disks will generally suffice, as these components will only have a minor impact on processing speed.”