Bioinspired preparation of polydopamine microcapsule for multienzyme system construction
By Lei Zhang, Jiafu Shi, Zhongyi Jiang, Yanjun Jiang, S. Qiao, Jian Li, Rui Wang, Ruijie Meng, Yuanyuan Zhu, Yang Zheng. 2011
159 citations recorded by the source corpus.
Inspired by the structural organization of mitochondria and the bioadhesive principle, a simple and versatile approach to construct a multienzyme system is developed. More specifically, the multienzyme system is composed of a polydopamine (PDA) microcapsule scaffold and three spatially separated enzymes. The PDA microcapsules are prepared through the rapid, spontaneous self-polymerization of dopamine on the surface of CaCO3 microparticle template, followed by dissolution of the template using EDTA. The wall thickness of the microcapsules can be tuned by the dopamine concentration in an aqueous solution. The three enzymes are respectively immobilized through physical encapsulation in the lumen, in situ entrapment within the wall and chemical attachment on the out surface under extremely mild conditions. As an example, a multienzyme system, containing α-amylase, β-amylase and glucosidase, was constructed to convert starch into isomaltooligosaccharide, and the multienzyme system displays higher catalytic activity and enhanced operational stability. The method developed in this study will establish a powerful platform for the facile construction of multienzyme cascade systems.
Subfields: Molecular Biology, Molecular Medicine, Surfaces, Coatings and Films
View Paper: https://www.semanticscholar.org/paper/564e6734030fea1c09774d91134b64dcd4ddbe73
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In Molecular Biology
Citations in the corpus
- Deposition Mechanism and Properties of Thin Polydopamine Films for High Added Value Applications in Surface Science at the Nanoscale (2011, methodology): “After dissolution of the CaCO3 core with EDTA, the enzymes kept their activity and allowed to create an enzymatic cascade [119].”