A T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase.

By Andrew D. Sharrocks. 1994

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1. Yimeng Yin
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8. Biswajyoti Sahu

Citations in the corpus

• Impact of cytosine methylation on DNA binding specificities of human transcription factors (2017, methodology): “5 was amplified from pDONR clone and cloned into NcoI-SacI restriction sites of pETGEXCT (67).”
• CG methylated microarrays identify a novel methylated sequence bound by the CEBPB|ATF4 heterodimer that is active in vivo (2013, methodology): “…the Pfam (Finn et al. 2008) and SMART (Letunic et al. 2004) database (Supplemental Table 7) were cloned into the pETGEXCT (C-terminal GST) vector (Sharrocks 1994) either by RT-PCR from pooled mouse mRNA (1 ng), followed by ligation-independent cloning, or by gene synthesis followed by…”