Thidiazuron-induced high-frequency direct shoot organogenesis of Cannabis sativa L.

By H. Lata, S. Chandra, I. Khan, M. Elsohly. In vitro cellular & developmental biology. Plant, 2009

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Thidiazuron: A multi-dimensional plant growth regulator (2011, background): “Liu et al. (2003) reported that cultures grown on medium supplemented with 1 μM TDZ for 20 days produced a maximum number of shoots (16) per intact seedling in Artemisia judaica L.”
A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.) (2016, background): “For example, apical nodal segments and shoot tips were reported as suitable explants for micropropagation and germplasm conservation, but not as common explants for genetic transformation (Bing et al., 2007; Lata et al., 2009; Wang et al., 2009).”
Cannabis sativa: The Plant of the Thousand and One Molecules (2016, methodology): “For example the addition of thidiazuron (TDZ), which has cytokinin-like activity, was shown to increase the development of shoots in hemp explants (Lata et al., 2009a) and in leaf-derived calli of a high yielding THCA clone (Lata et al., 2010).”