Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement

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Development of multiple SNP marker panels affordable to breeders through genotyping by target sequencing (GBTS) in maize (2019, background): “Compared to phenotypic selection, MAS has the following advantages (Tanksley et al. 1989; Xu and Crouch 2008): (1) MAS can shorten breeding cycles, improve breeding efficiency, and save a lot of labor and resources, and thus increase the total genetic gain per year (Xu et al. 2017a); (2) molecular…”
Single Nucleotide Polymorphism–based Genetic Diversity in the Reference Set of Peanut (Arachis spp.) by Developing and Applying Cost‐Effective Kompetitive Allele Specific Polymerase Chain Reaction Genotyping Assays (2013, background): “(Semagn et al., 2013).”
Genetic Gains in Grain Yield Through Genomic Selection in Eight Bi-parental Maize Populations under Drought Stress (2015, methodology): “com), while the three DTMA C0 populations were genotyped with 197 to 286 SNPs using a KASP assay (Semagn et al., 2014) at LGC genomics (www.”
High-Throughput SNP Genotyping to Accelerate Crop Improvement (2014, methodology): “More recently, the KASP™ competitive allele-specific PCR system has gained popularity for lower cost single-plex genotyping of crop species, for example with uptake in wheat and maize (Neelam et al. 2013; Semagn et al. 2014).”