176 A PREFORMED COMPLEX OF POSTSYNAPTIC PROTEINS IS INVOLVED IN EXCITATORY SYNAPSE DEVELOPMENT.
By K. Gerrow, Stefano Romorini, S. Nabi, C. Sala, A. El-Husseini. Journal of Investigative Medicine, 2006
222 citations recorded by the source corpus.
Nonsynaptic clusters of postsynaptic proteins has been previously documented; however, their role remains elusive. Here we monitored trafficking of several candidate proteins implicated in synaptogenesis, prior to the majority of synapse formation, and when nonsynaptic clusters of scaffold proteins are most abundant. We find that a protein complex consisting of two distinct subpopulations that differ in their content, mobility, and involvement in synapse formation. One subpopulation is mobile and relies on actin-dependent transport for delivery to nascent and existing synapses. These mobile clusters contain the scaffolding proteins PSD-95 (postsynaptic density-95), GKAP (guanylate kinase associated protein), and Shank but lack the cell adhesion molecule neuroligin-1. The second group consists of stationary nonsynaptic scaffold complexes that contain neuroligin-1 and recruit synaptophysin containing axonal transport vesicles. Sites with stationary complexes are readily transformed to functional presynaptic contacts that recycle the vital dye FM 4-64. These results postulate a novel mechanism whereby preformed scaffold protein complexes serve as predetermined postsynaptic hot spots for establishment of new functional excitatory synapses.
Subfields: Cell Biology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/52754e67ddc090326087e4548d3614b3ccc57347
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In Cell Biology
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In Molecular Biology
Citations in the corpus
- The cell biology of synaptic plasticity: AMPA receptor trafficking. (2007, background): “Early in neuronal development, packets of receptors and scaffolding proteins travel along dendrites (Gerrow et al. 2006, Washbourne et al. 2002).”
- Compartmentalizing the neuronal plasma membrane from axon initial segments to synapses. (2009, background): “Other groups have shown evidence for modular transport of PSD-95 (Prange and Murphy, 2001) and scaffold complexes containing PSD-95, Shank and GKAP (Gerrow et al., 2006) during synaptogenesis, similar to STVs and PTVs in axon.”
- Neurexin-neuroligin signaling in synapse development. (2007, background): “clusters in axons can precede apposing clusters of PSD-95 [50], and that PSD-95–neuroligin clusters on dendrites can precede apposing synaptic vesicle clusters [51].”
- Cell adhesion molecules: signalling functions at the synapse (2007, background): “14 ), an important component of the postsynaptic density that clusters other postsynaptic protein”
- Assembly of a β2‐adrenergic receptor—GluR1 signalling complex for localized cAMP signalling (2010, methodology): “Images were scaled to 16 bits and analysed in Northern Eclipse (Empix Imaging, Mississauga, Canada), by using user-written software routines (Gerrow et al, 2006; Lise et al, 2006).”