Computational structure-based redesign of enzyme activity

205 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

A general-purpose protein design framework based on mining sequence–structure relationships in known protein structures (2018, unknown): “Nevertheless, despite all of these developments, and many examples of successful designs in the literature (18, 26-35), it is still the case that CPD methods are not robust.”
Coupling Protein Side-Chain and Backbone Flexibility Improves the Re-design of Protein-Ligand Specificity (2015, background): “A common feature of the previous work in this area is the assumption that the protein backbone remains fixed in conformation during the sequence design step, although there are some exceptions [9,24].”
Achievements and Challenges in Computational Protein Design. (2017, unknown): “In 2009 the Donald lab conducted computational structurebased redesign of the phenylalanine adenylation domain of the nonribosomal peptide synthetase enzyme gramicidin S synthetase A (GrsA-PheA) for a set of noncognate substrates for which the wild-type enzyme has little or virtually no specificity ”
Beyond directed evolution - semi-rational protein engineering and design (2010, methodology): “Chen et al . used their K * algorithm to switch the substrate specificity of the phenylalanine adenylation domain in the nonribosomal peptide synthetase enzyme gramicidin S synthetase A [26].”