Doubly-Labelled Water – Theory and Practice

By R. Harris. 2001

377 citations recorded by the source corpus.

Subfields: Ecology, Nature and Landscape Conservation

View Paper: https://www.semanticscholar.org/paper/5051132aa4fc5b6ac620815d4c8c07fb00658bd2

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Citations in the corpus

Standard methods for physiology and biochemistry research in Apis mellifera (2013, methodology): “The doubly-labelled water technique is an appropriate method to measure field metabolic rates of free ranging insects (Wolf et al., 1996; Speakman, 1997, 1998).”
Effects of structural and functional habitat gaps on breeding woodland birds: working harder for less (2008, methodology): “The enrichment of the injectate was calculated for the five different solutions (Prentice 1990, Speakman 1997), and then averaged.”
Energy balance and its components: implications for body weight regulation. (2012, background): “Thus, the combined error of assessing energy imbalance can easily reach 1000 kcal/d (4200 kJ/d) (21).”
Measuring metabolic rate in the field: the pros and cons of the doubly labelled water and heart rate methods (2004, methodology): “© 2004 British Ecological Society, Functional Ecology , 18 , 168–183 A wide variety of approaches has been used to measure the energetic state of individual animals (Speakman 1997).”
Energy metabolism and animal personality (2008, methodology): “This technique consists of injecting an animal with DLW, and taking an initial, post-equilibration blood sample, then releasing the animal for a multi-day sampling period, before trying to re-capture the animal for a second blood sample (Speakman 1997).”
Feeling the heat: Australian landbirds and climate change (2012, background): “In addition, daily changes in hydration status should be measured by comparing total body water at various times of the day by injecting birds with isotopically labelled water and measuring the dilution space once the label has equilibrated with the body water pool (Speakman 1997).”
Food requirements of wild animals: predictive equations for free-living mammals, reptiles, and birds (2001, methodology): “…feeding rates were estimated from field metabolic rates, as measured using the doubly labeled water method (Lifson and McClintock, 1966; Nagy, 1983; Speakman, 1997) for the 229 species of terrestrial vertebrates summarized in the recent review by Nagy et al. (1999; see link…”
Accelerometers can measure total and activity-specific energy expenditures in free-ranging marine mammals only if linked to time-activity budgets (2017, methodology): “Heart rate monitors, accelerometers and doubly labelled water (DLW) have all been used to measure energy expenditure in vertebrates (Lifson & McClintock 1966; Butler et al. 1992; Butler 1993; Speakman 1997; Froget et al. 2004; Wilson et al. 2006; Young et al. 2011).”
Expenditure freeze: the metabolic response of small mammals to cold environments (2005, methodology): “Ecology Letters (2005) 8: 1326–1333”
Travelling to breed (2006, background): “Parental energetics during incubation can be assessed by using doubly-labelled water (Speakman 1997; Tinbergen and Williams 2002) requiring capture and recapture of the incubating parent(s).”
Body size, energy metabolism and lifespan (2005, methodology): “The DLW method is an isotope-based method for the measurement of whole body CO2 production and hence energy expenditure (Nagy, 1994, 2005; Speakman, 1997).”
What makes marine turtles go: A review of metabolic rates and their consequences (2008, methodology): “In contrast, fasted animals exhibited changing TBW pool size and greater reductions in MR than in water turnover, thus violating several assumptions of the DLW method (Speakman, 1997).”
Huddling in groups leads to daily energy savings in free‐living African Four‐Striped Grass Mice, Rhabdomys pumilio (2006, methodology): “The isotope ratios 18 O: 16 O and 2 H: 1 H were analysed using gas source isotope ratio mass spectrometry (Optima, Micromass IRMS and Isochrom µ G, Manchester, UK), prior to calculation of DEE (Lemen & Speakman 1997).”