Real-time selective sequencing using nanopore technology

By M. Loose, S. Malla, Michael Stout. Nature Methods, 2016

370 citations recorded by the source corpus.

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.

Subfields: Artificial Intelligence, Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4fd7d0af133a2bd564f52f9bb1268a1ec1d05014

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In Artificial Intelligence

1. Hengyun Lu
2. Zemin Ning
3. Francesca Giordano

In Biomedical Engineering

1. Hengyun Lu
2. Zemin Ning
3. J. de Ridder
4. Wigard P. Kloosterman
5. Francesca Giordano
6. Franka J. Rang

In Molecular Biology

1. Hengyun Lu
2. Zemin Ning
3. J. de Ridder
4. Wigard P. Kloosterman
5. Francesca Giordano
6. Franka J. Rang

Citations in the corpus

• Oxford Nanopore MinION Sequencing and Genome Assembly (2016, methodology): “To speed up data collection for analysis, Loose et al. [71] developed a method which can map the squiggle data directly to a reference sequence in ‘squiggle space’.”
• From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy (2018, background): “The pore models provide distributions of the mean signal and standard deviations that can be expected for each k-mer, while the scaling parameters help to correct for differences in signal that may occur between different wells or over the course of a sequencing run [36].”