New Technologies for Analysis of Extracellular Vesicles

By Huilin Shao, H. Im, Cesar M. Castro, X. Breakefield, Ralph Weissleder, Hakho Lee. Chemical Reviews, 2018

1,454 citations recorded by the source corpus.

Extracellular vesicles (EVs) are diverse, nanoscale membrane vesicles actively released by cells. Similar sized vesicles can be further classified (e.g., exosomes, microvesicles) based on their biogenesis, size and biophysical properties. Although initially thought to be cellular debris, and thus under-appreciated, EVs are now increasingly recognized as important vehicles of intercellular communication and circulating biomarkers for disease diagnoses and prognosis. Despite their clinical potential, the lack of sensitive preparatory and analytical technologies for EVs poses a barrier to clinical translation. New analytical platforms including molecular ones are thus actively being developed to address these challenges. Recent advances in the field are expected to have far-reaching impact in both basic and translational studies. This article aims to present a comprehensive and critical overview of emerging analytical technologies for EV detection, and their clinical applications.

Subfields: Cancer Research, Immunology and Allergy, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4fbd3317fd8a9220b18e33e9a10b46331ec05e3e

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Citations in the corpus

Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material (2019, neutral): “for single sEV analysis (reviewed in [18]).”
Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications (2020, neutral): “marker proteins, and is suitable for highthroughput analysis.(72) The operation is complicated and time-”
Exosomes as a new frontier of cancer liquid biopsy (2022, background): “Western blot and enzyme-linked immunosorbent assay (ELISA) are two regular approaches widely used to detect exosomal proteins [7, 107].”
Plant exosome like nanovesicles: Emerging therapeutics and drug delivery nanoplatforms. (2020, background): “Although earlier perceived to be cellular debris and hence undervalued, ELNVs are now acknowledged as crucial entities to regulate physiological functions of multicellular organisms in an intercellular transmission manner.(3) Despite a growing appreciation of the importance of ELNVs in mammals, and ”