Bacillus pumilus BpCRI 6, a promising candidate for cellulase production under conditions of catabolite repression

By O. S. Kotchoni, O. Shonukan, W. E. Gachomo. 2003

116 citations recorded by the source corpus.

Cellulose degrading organisms have been used for the conversion of cellulolytic materials into soluble sugars or solvents in several biotechnological and industrial applications. In this report, a mutant of Bacillus pumilus was obtained after chemical mutagenesis and screened for cellulase production. This mutant named BpCRI 6 was selected for its ability to produce cellulase under catabolite repression. Cellulase yield by BpCRI 6 was four times higher than that of the wild type under optimum growth conditions (pH 6.5, 25°C and Ca 2+ 1mM). In shaking flask cultures, production of cellulase by the wild type was completely repressed in the presence of 25 mM glucose, while BpCRI 6 strain still exhibited a residual cellulase production of 80 and 40% at 25 mM and 40 mM of glucose concentrations respectively. The mutant strain is stable and grows rapidly in liquid and solid media. Under conditions of catabolite repression (40 mM of glucose), the production of cellulase by this mutant is particularly significant when compared to Trichoderma reesei a well-known cellulase producer, which is under control of end-product inhibition. This is the first report of a successful catabolite repression insensitivity of cellulase production by a mutant of B. pumilus.

Subfields: Biomedical Engineering, Biotechnology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4ecbd05f942e69afaa4ac95719f470edea4426e0

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In Biomedical Engineering

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In Biotechnology

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In Molecular Biology

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3. S. Howard
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Citations in the corpus

• Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw (2006, background): “Cellulase was identified as one of the key enzyme degrading cellulose (Kotchoni et al., 2003).”
• Lignocellulose biotechnology: issues of bioconversion and enzyme production (2003, background): “The first catabolite repressed Bacillus pumilus with cellulase yield four times higher than the wild type strain was created through mutagenesis (Kotchoni et al. 2003).”
• Production and characterisation of cellulase from Bacillus pumilus EB3 (2006, background): “The high temperature for optimum endoglucanase activity is also an advantage over fungal cellulase as common cellulase from fungus, namely Trichoderma and Aspergillus sp. has an optimum temperature of 40 - 55°C [13, 17-18 ].”
• Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives (2008, background): “lase yields four times higher than the wild type strain that was created through mutagenesis [56].”