High-throughput tissue extraction protocol for NMR- and MS-based metabolomics.
By Huifeng Wu, A. Southam, A. Hines, Mark R. Viant. Analytical Biochemistry, 2008
624 citations recorded by the source corpus.
Subfields: Molecular Biology, Physiology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/4eaf04d437810238577ff3ca0b80ca21855ebc63
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Robert Powers
- Bradley Worley
- Myrna J. Simpson
- Edward G. Nagato
- Brian P. Lankadurai
- Royston Goodacre
- Mark R. Viant
- David E. Neal
In Spectroscopy
- Myrna J. Simpson
- Edward G. Nagato
- Brian P. Lankadurai
- Royston Goodacre
- Robert Powers
- Bradley Worley
- Mark R. Viant
Citations in the corpus
- Evaluation of extraction processes for intracellular metabolite profiling of mammalian cells: matching extraction approaches to cell type and metabolite targets (2010, methodology): “This method was adapted from Wu et al. (2008).”
- A new approach to toxicity testing in Daphnia magna: application of high throughput FT-ICR mass spectrometry metabolomics (2009, methodology): “Metabolites were extracted from whole D. magna using the two step methanol:water:chloroform (2:2:1.8 final solvent ratio) protocol as described previously ( Wu et al. 2008 ).”
- The androgen receptor fuels prostate cancer by regulating central metabolism and biosynthesis (2011, methodology): “…FBS (steroid depleted conditions) and treated either with vehicle (0.01% ethanol), androgen (1 nM R1881), androgenþ STO609 (25mM) or androgenþCAMKK2 siRNA for 72 h. Cells were harvested by scraping on ice and metabolites were obtained by methanol/chloroform extraction (Wu et al, 2008).”
- Multivariate Analysis in Metabolomics. (2012, background): “Mass spectrometry is similarly employed for metabolic fingerprinting and is typically favored for its sensitivity in more global metabolic profiling applications, but generally requires upstream chromatographic separations due to the limited molecular-weight diversity of metabolites [37-39].”
- Environmental metabolomics: an emerging approach to study organism responses to environmental stressors (2013, background): “The choice of tissue/organ extraction method is also a critical step in metabolomic analyses (Lin et al. 2007; Wu et al. 2008; Beltran et al. 2012).”