Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice
By B. Trouiller, R. Reliene, Aya M. Westbrook, Parrisa Solaimani, R. Schiestl. Cancer Research, 2009
762 citations recorded by the source corpus.
Titanium dioxide (TiO2) nanoparticles (NPs) are manufactured worldwide in large quantities for use in a wide range of applications including pigment and cosmetic manufacturing. Although TiO2 is chemically inert, TiO2 NPs can cause negative health effects, like respiratory tract cancer in rats. However, the mechanisms involved in TiO2-induced genotoxicity and carcinogenicity have not been clearly defined and are poorly studied in vivo. The present study investigates TiO2 NP-induced genotoxicity, oxidative DNA damage and inflammation in a mice model. We treated wild type mice with TiO2 NPs in drinking water and determined the extent of DNA damage using the comet assay, the micronuclei assay, the γ-H2AX immuno-staining assay and by measuring 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels and, as genetic instability end point, DNA deletions. We also determined mRNA levels of inflammatory cytokines in the peripheral blood. Our results show that TiO2 NPs induced 8-OHdG, γ-H2AX foci, micronuclei and DNA deletions. The formation of γ-H2AX foci, indicative of DNA double strand breaks, was the most sensitive parameter. Inflammation was also present as characterized by a moderate inflammatory response. Together these results describe the first comprehensive study of TiO2 NP induced genotoxicity in vivo in mice, possibly caused by a secondary genotoxic mechanism associated with inflammation and/or oxidative stress. Given the growing use of TiO2 NPs, these findings raise concern about potential health hazards associated with TiO2 NP exposure.
Subfields: Health, Toxicology and Mutagenesis, Materials Chemistry
View Paper: https://www.semanticscholar.org/paper/4e0bfa31bbd05c39bfdad14d2eb84b187d554251
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In Health, Toxicology and Mutagenesis
In Materials Chemistry
Citations in the corpus
- Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay (2014, background): “, 2004) and TiO2 NPs induce DNA damage and genetic instability in mice (Trouiller et al., 2009).”
- Toxicity assessment of nanomaterials: methods and challenges (2010, background): “The harmful effects of nanoparticles have also been studied in vivo [15–18].”
- Titanium dioxide nanoparticles: a review of current toxicological data (2013, background): “Furthermore, most studies were not designed to investigate the relationship between TiO2 particle size and lung cancer risk, which represents an important question for assessing the potential occupational carcinogenicity of TiO2 NPs [32].”