Systematic mutation of bacteriophage T4 lysozyme.

By D. Rennell, S. Bouvier, L. Hardy, A. Poteete. 1991

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Citations in the corpus

Robustness and evolvability in the functional anatomy of a PER-ARNT-SIM (PAS) domain (2010, background): “Such studies typically evaluate the effects of mutations through in vivo processes that are easily detected, such as plaque formation (8) and antibiotics resistance (9).”
Evolvability as a function of purifying selection in TEM-1 β-lactamase. (2015, unknown): “The tolerance of many proteins to mutations has been assessed in a number of important studies with high-throughput site-directed and random mutagenesis (Fowler et al., 2010; Guo et al., 2004; Huang et al., 1996; Jacquier et al., 2013; Loeb et al., 1989; McLaughlin et al., 2012; Melnikov et al., 201”
Systematic exploration of ubiquitin sequence, E1 activation efficiency, and experimental fitness in yeast (2014, background): “While a wealth of studies demonstrate that the fitness effects of mutations are mediated by biochemical changes 19; 20; 21; 22; 23; 24; 25 , most systematic studies of mutants have focused predominantly on either growth effects 14; 26; 27 or biochemical effects 11; 28; 29; 30 .”
Needles in stacks of needles: finding disease-causal variants in a wealth of genomic data (2011, unknown): “One such source is mutagenesis experiments of individual proteins that generate many possible mutations and assess their function, as has been performed for several bacterial and viral protein”
Neutral and weakly nonneutral sequence variants may define individuality (2013, background): “Over 4,000 mutants in Lac Ι repressor and over 2,000 in bacteriophage T4 lysozyme ( “ L&L ” ) were experimentally evaluated for functional impact (13, 14).”
SIFT missense predictions for genomes (2015, methodology): “Step 2: Top 5,000 hits aligned by Smith-Waterman algorithm using SW#DB We used four different data sets to assess performance: HumDiv, HumVar, LacI and lysozyme 28–30 .”
News from the protein mutability landscape. (2013, background): “[61] Rennell D, Bouvier SE, Hardy LW, Poteete AR.”
Network analysis of protein structures identifies functional residues. (2004, background): “The positions where mutations effected protein activity were identiﬁed directly in T4-lysozyme and barnase, 29,32 and indirectly in TEM1-b -lactamase (TEM1).”
Latent Effects of Hsp90 Mutants Revealed at Reduced Expression Levels (2013, background): “For example, the net function of lysozyme in phage T4 must be reduced about 30-fold before experimentally measurable impacts on growth are observed [13].”