High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

By Traver Hart, M. Chandrashekhar, Michael Aregger, Z. Steinhart, K. Brown, G. Macleod, Monika Miś, Michal Zimmermann, Amélie Fradet-Turcotte, Song Sun and 8 more. Cell, 2015

1,489 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4cd832d8101c16281159987894f056d39c22406f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

In Molecular Biology

Citations in the corpus

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens (2017, methodology): “From the base 90k TKOv1 library (Hart et al. 2015), we identified genes in the new CEG2 set that were targeted by six gRNAs each. gRNAs were ranked by log foldchange, and the three gRNAs with the best (most negative) fold-change were identified, as well as the worst (remaining three gRNA).”
A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia (2016, background): “Recent reports on CRISPR screens on several cancer cell lines have demonstrated their power (Hart et al., 2015; Wang et al., 2015).”
Computational correction of copy-number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells (2017, background): “The depletion ranks of the 100 genes with the largest CN measurements were significantly higher than expected for the majority of cell lines (300/342 with FDR-corrected p < 0.05, one-sample one-tailed K-S test; Fig.”
DeepCRISPR: optimized CRISPR guide RNA design by deep learning (2018, background): “The initial on-target dataset contains seed sgRNAs with experimentally validated known knockout efficacy, comprising ~ 15,000 sgRNAs containing 1071 genes from four different cell lines ( hct116 [36], hek293t [2], hela [36], and hl60 [37]) with redundancy removed.”
Uncovering the essential genome of the human malaria parasite Plasmodium falciparum by saturation mutagenesis (2018, positive): “falciparum, we developed high-throughput transfection mutagenesis methods that mostly create a single insertion per genome and combined them with an Illumina-based sequencing method for identifying transposon insertion sites (17, 18).”
Evaluation and Design of Genome-wide CRISPR/Cas9 Knockout Screens (2017, background): “Traver Hart[1]*, Amy Tong [2], Katie Chan [2], Jolanda Van Leeuwen [2], Ashwin Seetharaman [2], Michael Aregger [2], Megha Chandrashekhar [2], Nicole Hustedt[3], Sahil Seth[4], Avery Noonan [2], Andrea Habsid [2], Olga Sizova [2], Lyudmila Nedyalkova [2], Ryan Climie [2], Keith Lawson [2], Maria Aug”
CRISPRi-based genome-scale identification of functional long non-coding RNA loci in human cells (2016, neutral): “8% being essential to all cell types screened in (47) and (46), respectively (Fig.”