Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples.

By A. Parada, D. Needham, Jed A. Fuhrman. Environmental Microbiology, 2016

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Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys (2015, background): “(4) 806r Modified GGACTACNVGGGTWTCTAAT Apprill et al.”
Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels (2017, background): “Both forward (515fB) and reverse (806rB) primers for the 16S rRNA gene, targeting microorganisms, were tagged yielding dual-indexed reads (Kozich et al., 2013; Apprill et al., 2015; Parada et al., 2016; Table 1).”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, background): “However, some Archaea, particularly thermophiles, contain 16S rRNA gene introns in otherwise highly conserved regions, which may impact the annealing of some ‘universal’ primers used to target this gene (Parada et al. 2015).”
Linking meta-omics to the kinetics of denitrification intermediates reveals pH-dependent causes of N2O emissions and nitrite accumulation in soil (2020, background): “7 the 16S, 515f and 806rB [49, 50], as detailed by the Earth Microbiome project 198 (http://www.”