A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma

By Maolei Zhang, Kun Zhao, Xiaoping Xu, Yibing Yang, Sheng Yan, P. Wei, Hui Liu, Jianbo Xu, Feizhe Xiao, Huangkai Zhou and 8 more. Nature Communications, 2018

613 citations recorded by the source corpus.

Circular RNAs (circRNAs) are a large class of transcripts in the mammalian genome. Although the translation of circRNAs was reported, additional coding circRNAs and the functions of their translated products remain elusive. Here, we demonstrate that an endogenous circRNA generated from a long noncoding RNA encodes regulatory peptides. Through ribosome nascent-chain complex-bound RNA sequencing (RNC-seq), we discover several peptides potentially encoded by circRNAs. We identify an 87-amino-acid peptide encoded by the circular form of the long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) that suppresses glioblastoma cell proliferation in vitro and in vivo. This peptide directly interacts with polymerase associated factor complex (PAF1c) and inhibits the transcriptional elongation of multiple oncogenes. The expression of this peptide and its corresponding circRNA are decreased in glioblastoma compared with the levels in normal tissues. Our results establish the existence of peptides encoded by circRNAs and demonstrate their potential functions in glioblastoma tumorigenesis. Functional peptides can be encoded by short open reading frames in non-coding RNA. Here, the authors identify a 87aa peptide encoded by the circular form of the long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) that can reduce glioblastoma proliferation via interaction with PAF1 which sequentially inhibits the transcriptional elongation of some oncogenes.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4cd47201abdd67cb77c74fe7d2e65d6f966f1bdc

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Wanying Wu
2. Peifeng Ji
3. Fangqing Zhao

In Molecular Biology

1. Wanying Wu
2. Peifeng Ji
3. Fangqing Zhao

Citations in the corpus

• CircAtlas: an integrated resource of one million highly accurate circular RNAs from 1070 vertebrate transcriptomes (2020, background): “In addition, several recent studies have reported that the circRNAs can encode proteins and their translated products may play important roles in various biological processes [13 – 16].”
• Circular RNA-protein interactions: functions, mechanisms, and identification (2020, background): “circPINTexon2 tumour inhibitor cancer cell Cytoplasm [102]”
• Non-coding RNAs in disease: from mechanisms to therapeutics (2023, neutral): “Indeed, several circRNA-encoded proteins, such as FBXW7-185aa, SHPRH-146aa and PINT-87aa, were shown to suppress glioma tumorigenesis 85,132,133 .”