Structures of the mannose-6-phosphate pathway enzyme, GlcNAc-1-phosphotransferase

By A. Gorelik, K. Illes, K. Bui, B. Nagar. 2022

14 citations recorded by the source corpus.

Significance Lysosomes are cellular organelles containing a variety of degradative enzymes. The protein N-acetylglucosamine-1-phosphotransferase (GNPT) is responsible for directing these enzymes to be delivered to lysosomes. Genetic mutations in GNPT cause the diseases mucolipidosis types II and III. In order to better understand the function of GNPT at the molecular level, we determined the three-dimensional structures of its catalytic domain and of its sugar-binding subunit. These structures, along with biochemical experiments, reveal its enzymatic mechanism and its sugar recognition mode.

Subfields: Organic Chemistry, Physiology

Citations in the corpus

• Generalized Biomolecular Modeling and Design with RoseTTAFold All-Atom (2023, neutral): “3F) and have multiple mono-saccharides and different branching patterns ( 41 , 42 ).”