Development of PVS-Based Vitrification and Encapsulation–Vitrification Protocols

By A. Sakai, D. Hirai, T. Niino. 2008

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The Application of Biotechnology to Orchids (2013, background): “This procedure has been developed for meristem, cell suspensions and somatic embryos of numerous different species (Sakai and Engelmann, 2007; Sakai et al., 2008).”
Cryopreservation of Phytodiversity: A Critical Appraisal of Theory & Practice (2008, background): “…cryoprotective strategies, an approach that has proved highly successful, particularly in the development of plant vitrification-based strategies (Sakai et al., 2008), leading to significant successes in the largescale application of plant cryopreservation, especially to tropical crops…”
Use of biotechnologies for the conservation of plant biodiversity (2011, methodology): “It has been applied to apices of an increasing number of species (Sakai and Engelmann 2007; Sakai et al. 2008).”
Current Issues in Plant Cryopreservation (2012, methodology): “The most commonly used mixture of CPAs for vitrification is plant vitrification solution 2 (PVS2), which consists of 30% (w/v) glycerol, 15% (w/v) ethylene glycol and 15% (w/v) DMSO in basal culture medium containing 0.4 M sucrose (Sakai et al., 1990, 2008).”