Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry

By S. V. Kamzolova, A. Fatykhova, E. G. Dedyukhina, S. Anastassiadis, N. P. Golovchenko, I. Morgunov. 2011

86 citations recorded by the source corpus.

Summary The possibility of using glycerol and glycerol-containing waste from biodiesel manufacture as a carbon and energy source for microbiological production of citric acid has been studied. Acid formation on the selective media had previously been tested in 66 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis and Yarrowia). Under growth limitation by nitrogen, 41 strains (belonging mainly to species Yarrowia lipolytica) produced acids; unlike 25 strains of the genera Debaryomyces, Candida, Pichia, Saccharomyces and Torulopsis. Among the 41 acid-producing strains, mutant strain Yarrowia lipolytica N15 was selected since it was able to produce citric acid presumably in high amounts. The citric acid production by the selected strain was studied in dependence on the medium pH, aeration and concentration of glycerol. Under optimal conditions, the mutant Y. lipolytica N15 produced up to 98 g/L of citric acid when grown in a fermentor with the medium containing pure glycerol, and 71 g/L of citric acid when grown on glycerol-containing waste. The effect of growth phases on physiological peculiarities of the citric acid producer was discussed.

View Paper: https://www.semanticscholar.org/paper/4957142a7bedd34226c6dba1186d053de5a9cbdd

Citations in the corpus

• Value-added uses for crude glycerol--a byproduct of biodiesel production (2012, unknown): “lipolytica N15 could produce citric acid in high amounts, specifically, up to 98 g/L of citric acid and 71 g/L of citric acid were produced from pure glycerol medium and crude glycerol medium, respectively [43].”