Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina
By J. V. Wagenen, T. Miller, S. Hobbs, Paul W. Hook, B. Crowe, M. Huesemann. 2012
247 citations recorded by the source corpus.
Accurate prediction of algal biofuel yield will require empirical determination of physiological responses to the environment, particularly light and temperature. One strain of interest, Nannochloropsis salina , was subjected to ranges of light intensity (5–850 μmol m −2 s −1 ) and temperature (13–40 °C) and its exponential growth rate, total fatty acids (TFA) and fatty acid composition were measured. The maximum acclimated growth rate was 1.3 day −1 at 23 °C and 250 μmol m −2 s −1 . Fatty acids were detected by gas chromatography with flame ionization detection (GC-FID) after transesterification to corresponding fatty acid methyl esters (FAMEs). A sharp increase in TFA containing elevated palmitic acid (C16:0) and palmitoleic acid (C16:1) during exponential growth at high light was observed, indicating likely triacylglycerol accumulation due to photo-oxidative stress. Lower light resulted in increases in the relative abundance of unsaturated fatty acids; in thin cultures, increases were observed in palmitoleic and eicosapentaenoic acids (C20:5ω3). As cultures aged and the effective light intensity per cell converged to very low levels, fatty acid profiles became more similar and there was a notable increase of oleic acid (C18:1ω9). The amount of unsaturated fatty acids was inversely proportional to temperature, demonstrating physiological adaptations to increase membrane fluidity. These data will improve prediction of fatty acid characteristics and yields relevant to biofuel production.
Subfields: Oceanography, Renewable Energy, Sustainability and the Environment
View Paper: https://www.semanticscholar.org/paper/488c04a40513bb879e01dce8bd341b3a1a2a4e01
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Citations in the corpus
- Trends and strategies to enhance triacylglycerols and high-value compounds in microalgae (2017, background): “DHA contents in several mesophilic species [50,55,56].”
- A validated model to predict microalgae growth in outdoor pond cultures subjected to fluctuating light intensities and water temperatures (2016, background): “sorokiniana (DOE 1412), the maximum specific growth rate was also measured in dilute cultures as a function of light intensity ranging from 44 to 1950 μmol/m(2)·s [45].”
- Algal Biofuels: Current Status and Key Challenges (2019, background): “The quantity of unsaturated fatty acids decreases with increasing the temperature which is a physiological adaptation [21].”
- Factors Affecting Growth of Various Microalgal Species (2018, unknown): “…of 370, 1,850, 3,700, 7,400, 18,500, and 62,900 lux (5, 25, 50, 100, 250, and The growth rate increased with light intensity and the highest growth rate was achieved at 18,500 lux; however, photon conversion efficiency decreased for light efficiencies > 3,700 lux (Van Wagenen et al. , 2012).”