Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques

By R. Amann, Bernhard Fuchs. Nature Reviews Microbiology, 2008

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How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Microbial consortia of bacteria and fungi with focus on the lichen symbiosis (2009, positive): “Unprecedented progress has been achieved with the application of new molecular and microscopic techniques (Smalla, 2004; Allen and Banfield, 2005; Amann and Fuchs, 2008).”
Peracetic acid degradation and effects on nitrification in recirculating aquaculture systems. (2009, methodology): “For culture-independent identification and enumeration of ammonia- and nitrite-oxidizing Bacteria and Archaea in biofilm samples collected during the experimental period the fluorescence in situ hybridisation (FISH) technique (Amann and Fuchs, 2008) was applied.”
DECIPHER, a Search-Based Approach to Chimera Identification for 16S rRNA Sequences (2011, methodology): “The small subunit (SSU) rRNA molecule has been used extensively as a phylogenetic marker since the late 1980s (18), and nowadays, 16S rRNA sequences are essential for microbial identification (2, 19).”
Standard methods for research on Apis mellifera gut symbionts (2013, background): “…tract FISH with fluorescently labelled oligonucleotides targeting bacterial rRNAs can be a very powerful tool for detecting specific bacteria in various environmental samples such as active sludge, stool, clinical specimens, animal tissues and so on (Wagner et al. 2003, Amann and Fuchs 2008).”