Guidelines and considerations for the use of system suitability and quality control samples in mass spectrometry assays applied in untargeted clinical metabolomic studies
By David Broadhurst, Royston Goodacre, S. Reinke, J. Kuligowski, Ian A. Wilson, M. Lewis, Warwick B. Dunn. Metabolomics, 2018
709 citations recorded by the source corpus.
BackgroundQuality assurance (QA) and quality control (QC) are two quality management processes that are integral to the success of metabolomics including their application for the acquisition of high quality data in any high-throughput analytical chemistry laboratory. QA defines all the planned and systematic activities implemented before samples are collected, to provide confidence that a subsequent analytical process will fulfil predetermined requirements for quality. QC can be defined as the operational techniques and activities used to measure and report these quality requirements after data acquisition.Aim of reviewThis tutorial review will guide the reader through the use of system suitability and QC samples, why these samples should be applied and how the quality of data can be reported.Key scientific concepts of reviewSystem suitability samples are applied to assess the operation and lack of contamination of the analytical platform prior to sample analysis. Isotopically-labelled internal standards are applied to assess system stability for each sample analysed. Pooled QC samples are applied to condition the analytical platform, perform intra-study reproducibility measurements (QC) and to correct mathematically for systematic errors. Standard reference materials and long-term reference QC samples are applied for inter-study and inter-laboratory assessment of data.
Subfields: Food Science, Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/4787a5ce29b545c958edd5e5534b13c7f53b2d62
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Food Science
In Molecular Biology
In Spectroscopy
Citations in the corpus
- Metabolomics for Investigating Physiological and Pathophysiological Processes. (2019, methodology): “Regardless of whether one uses targeted or untargeted metabolomics, both methods require the use of internal standards and quality control (QC) samples (55, 128, 508).”