STRAIN IDENTIFICATION OF SPODOPTERA FRUGIPERDA (LEPIDOPTERA: NOCTUIDAE) INSECTS AND CELL LINE: PCR-RFLP OF CYTOCHROME OXIDASE C SUBUNIT I GENE

By Hazel C Levy, A. Garcia-Maruniak, J. Maruniak. 2002

156 citations recorded by the source corpus.

Abstract A simple method was developed to analyze the two morphologically indistinguishable host-associated strains of the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith). Total DNA extracted from the FAW corn and rice strains, as well as from the S. frugiperda cell line (SF9) was used to PCR amplify a 569 base pairs region of the mitochondrial gene cytochrome oxidase subunit I (COI). The amplified DNA from the Spodoptera corn strain and the SF9 cell line contained a restriction fragment length polymorphism (RFLP) marker, the MspI recognition site that was not present in the rice strain. This PCR-RFLP method does not require purification of mitochondrial DNA (mtDNA) or the use of radioactive isotopes, and differs from previous methods in that only a few nanograms of total DNA are needed to yield clear and accurate strain identification of individual insects.

Subfields: Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/475b7aae705d0a33fb4d8efe02989c4c0f492701

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In Insect Science

1. R. Nagoshi
2. R. Meagher

In Molecular Biology

1. R. Nagoshi
2. R. Meagher

Citations in the corpus

• Identification and Comparison of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Brazil, Texas, and Florida (2007, methodology): “…of 258, 564, and 600 indicate nucleotide distance from the presumptive translational start site of the COI gene) were described previously in sequence, and restriction enzyme analyses of an internal 568-bp region was deÞned by the JM76/JM77 primers (Levy et al. 2002, Nagoshi et al. 2005).”
• Seasonal Distribution of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Agricultural and Turf Grass Habitats (2004, methodology): “To determine strain identity from individual moths, genomic DNA from a randomly selected subset (n 10) of the captured samples was tested by PCR for the mitochondrial COI gene restriction fragment-length polymorphism as described previously (Levy et al. 2002, Nagoshi and Meagher 2003).”
• Population dynamics and occurrence of Spodoptera frugiperda host strains in southern Florida (2004, methodology): “They included JM76, 50-GAGCTGAATTAGG(G/A) ACTCCAGG-30, and JM77, 50-ATCACCTCC(A/T)CCTGCAGGATC-30, which span the mitochondrial cytochrome oxidase C subunit I gene (COI) (Levy et al., 2002).”
• The Fall Armyworm Triose Phosphate Isomerase (Tpi) Gene as a Marker of Strain Identity and Interstrain Mating (2010, background): “Mitochondrial haplotypes are among the most convenient of these as they can be assayed by polymerase chain reaction (PCR) ampliÞcation of individual samples (Levy et al. 2002).”
• Review of Fall Armyworm (Lepidoptera: Noctuidae) Genetic Complexity and Migration (2009, background): “This supports a relatively ancient divergence of the 2 strains (Lewter et al. 2006), and further suggests that the factors driving divergence is present throughout most of the western hemisphere.”
• BEHAVIOR AND DISTRIBUTION OF THE TWO FALL ARMYWORM HOST STRAINS IN FLORIDA (2004, methodology): “In particular, an MspI restriction enzyme polymorphism was identified that was diagnostic of strain identity and for which a PCR-based detection method was developed (Levy et al. 2002; Lu & Adang 1996).”