A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer
By J. Wagner, M. Rapsomaniki, Stéphane Chevrier, T. Anzeneder, C. Langwieder, August Dykgers, M. Rees, A. Ramaswamy, S. Muenst, S. Soysal and 8 more. Cell, 2019
664 citations recorded by the source corpus.
Summary Breast cancer is a heterogeneous disease. Tumor cells and associated healthy cells form ecosystems that determine disease progression and response to therapy. To characterize features of breast cancer ecosystems and their associations with clinical data, we analyzed 144 human breast tumor and 50 non-tumor tissue samples using mass cytometry. The expression of 73 proteins in 26 million cells was evaluated using tumor and immune cell-centric antibody panels. Tumors displayed individuality in tumor cell composition, including phenotypic abnormalities and phenotype dominance. Relationship analyses between tumor and immune cells revealed characteristics of ecosystems related to immunosuppression and poor prognosis. High frequencies of PD-L1+ tumor-associated macrophages and exhausted T cells were found in high-grade ER+ and ER− tumors. This large-scale, single-cell atlas deepens our understanding of breast tumor ecosystems and suggests that ecosystem-based patient classification will facilitate identification of individuals for precision medicine approaches targeting the tumor and its immunoenvironment.
Subfields: Biophysics, Molecular Biology, Oncology
View Paper: https://www.semanticscholar.org/paper/4580475bb9a7501e217eaa7e4fd386257ca30d51
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
In Oncology
Citations in the corpus
- Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer. (2020, background): “In recent years, scRNA-seq appeared as a revolutionary technic for addressing intra-tumor cellular complexity (27,30,34-44).”
- The history and advances in cancer immunotherapy: understanding the characteristics of tumor-infiltrating immune cells and their therapeutic implications (2020, background): “TAMs harbor highly plastic phenotypes and can be induced to polarize toward classically activated (M1) or alternatively activated (M2) phenotypes to exert immunostimulatory or immunosuppressive functions in tumor immunity.207,208 M1 macrophages exhibit a proinflammatory phenotype with tumor-killing ”
- Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma (2020, background): “Single-cell studies of breast and lung tumors including non-malignant tissue found a majority of tumor cells did not resemble normal counterparts, possibly indicating a distinct paradigm in those tissues (Lambrechts et al., 2018; Wagner et al., 2019).”