CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
By Janice S. Chen, E. Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, J. Palefsky, Jennifer A. Doudna. Science, 2018
3,718 citations recorded by the source corpus.
Subfields: Aging, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/45754ebe2eec796185468122a2e19ef928f0928e
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
- Joy Y. Wang
- Bijoya Paul
- Guillermo Montoya
- Guozhen Liu
- Katherine S. Pollard
- Shiyuan Li
- Jin Wang
- Yi Li
Citations in the corpus
- HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation. (2019, background): “(3) Determination of the sensitivity for target RNA”
- CRISPR-Cas12a: Functional overview and applications (2020, background): “This activity is displayed by all Cas12a orthologs and degrades any available ssDNA molecule into single/double nucleotides [40].”
- CRISPR technology: A decade of genome editing is only the beginning (2023, background): “Some of these enzymes have also been harnessed for the development of imaging methods (18–20) and diagnostic approaches (21, 22).”
- Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy (2020, positive): “The conversion of amplified DNA back into RNA can be avoided by using the DNA-sensing Cas12 for detection, a method called ‘‘DETECTR’’ (Chen et al., 2018), which has recently been adapted for SARS-CoV-2 detection (Broughton et al., 2020).”
- Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities (2020, neutral): “Lately, LFAs have been adapted for nucleic acid testing, nucleic acid analysis and gene identification (Corstjens et al., 2001; Mao et al., 2009).”
- CRISPR/Cas Systems towards Next-Generation Biosensing. (2019, methodology): “Cas-mediated reaction is the key step in CRISPR/Cas biosensing, and the assay time is 15 min in HOLMES, 30 min in HOLMESv2, 1 h in DETECTR, and 30 min to 3 h in SHERLOCK, mostly depending on the Cas effectors selected.”