Injectable In Situ Forming Biodegradable Chitosan-Hyaluronic acid Based Hydrogels for Cartilage Tissue Engineering

By H. Tan, C. Chu, K. Payne, K. Marra. Biomaterials, 2009

948 citations recorded by the source corpus.

Injectable, biodegradable scaffolds are important biomaterials for tissue engineering and drug delivery. Hydrogels derived from natural polysaccharides are ideal scaffolds as they resemble the extracellular matrices of tissues comprised of various glycosaminoglycans (GAG). Here, we report a new class of biocompatible and biodegradable composite hydrogels derived from water-soluble chitosan and oxidized hyaluronic acid upon mixing, without the addition of a chemical crosslinking agent. The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of polysaccharide derivatives. In the current work, N-succinyl-chitosan (S-CS) and aldehyde hyaluronic acid (A-HA) were synthesized for preparation of the composite hydrogels. The polysaccharide derivatives and composite hydrogels were characterized by FTIR spectroscopy. The effect of the ratio of S-CS and A-HA on the gelation time, microstructure, surface morphology, equilibrium swelling, compressive modulus, and in vitro degradation of composite hydrogels was examined. The potential of the composite hydrogel as an injectable scaffold was demonstrated by encapsulation of bovine articular chondrocytes within the composite hydrogel matrix in vitro. The results demonstrated that the composite hydrogel supported cell survival and the cells retained chondrocytic morphology. These characteristics provide a potential opportunity to use the injectable, composite hydrogels in tissue engineering applications.

Subfields: Cell Biology, Molecular Medicine, Rheumatology

View Paper: https://www.semanticscholar.org/paper/454b747a41ad01a32e7b6ed486956223ebfaa13e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Medicine

1. H. Tan
2. E. Alvarenga
3. N. Bhattarai
4. J. Gunn
5. Miqin Zhang
6. Jinchen Sun

In Rheumatology

1. Miqin Zhang

Citations in the corpus

• Fundamental Concepts of Hydrogels: Synthesis, Properties, and Their Applications (2020, background): “It was observed that the compressive modulus, which is an important factor for cartilage tissue engineering, improved with an increasing amount of N-succinyl chitosan in the hybrid hydrogel [83].”
• Characterization and Properties of Chitosan (2011, background): “Hydrogels are attractive candidates for tissue regeneration due to its extracellular matrix mimic structure and the ability to form under mild conditions (Tan et al., 2009).”
• Alginate-Based Biomaterials for Regenerative Medicine Applications (2013, background): “In addition to gelatin, other biopolymers with amino groups such as chitosan and collagen can be employed for the Schiff-base linkage with oxidized alginate [61–63].”
• Chitosan-based hydrogels for controlled, localized drug delivery. (2010, background): “Schiff bases were formed between the polymers when N-succinylated chitosan and aldehyde-terminated hyaluronic acid were mixed together at physiological pH for 1–4 min [93].”