A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

By Sheila Fisher, Andrew Barry, Justin Abreu, Brian Minie, Jillian Nolan, T. Delorey, Geneva Young, Timothy J Fennell, Alexander Allen, L. Ambrogio and 17 more. 2011

533 citations recorded by the source corpus.

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

Subfields: Molecular Biology

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Citations in the corpus

Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture (2012, methodology): “First, they allow parallelization of the procedure in a way that is impossible using column-based methods (Farias-Hesson et al. 2010; Lennon et al. 2010; Lundin et al. 2010; Meyer and Kircher 2010; Fisher et al. 2011).”
Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels (2012, methodology): “In the second experiment we incorporated 12 EDITTAG indexed adapters and 12 Illumina TruSeq indexed adapters to DNA libraries using a modified version of an on-bead library preparation method [51] and reagents from New England Biolabs.”