Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

By C. Freudiger, Wei Min, B. Saar, Sijia Lu, G. Holtom, Chengwei He, Jason C. Tsai, J. Kang, Xiaohui S. Xie. Science, 2008

1,939 citations recorded by the source corpus.

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.

Subfields: Analytical Chemistry, Biophysics

View Paper: https://www.semanticscholar.org/paper/451778532d65dfaa0badbcf4226bdea0fa92f6d8

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Analytical Chemistry

1. Ji‐Xin Cheng
2. Xiaohui S. Xie

In Biophysics

1. Ji‐Xin Cheng
2. Xiaohui S. Xie

Citations in the corpus

• Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine (2015, background): “group reported high-speed, high-sensitivity singlefrequency SRS imaging by megahertz modulation of a picosecond laser andmodulation transfer to the other laser (20), and this work was quickly”
• Femtosecond stimulated Raman microscopy (2007, methodology): “Non-linear Raman comes in many “flavors”, the most prominent being coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS).”