Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation

By H. N. Murthy, Eun-Jung Lee, Kee Yoeup Paek. Plant Cell Tissue and Organ Culture, 2014

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Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review (2016, background): “Plant secondary metabolites are unique sources of pharmaceuticals, food additives, flavors and industrially important biochemicals [2].”
Light quality in plant tissue culture: does it matter? (2018, background): “…studies are still restricted to ex vitro culture conditions, but in vitro cultivation has many advantages, such as fewer external factors, control of the cultivation environment, and the possibility to use elicitors for the metabolic pathway of interest (Hussain et al. 2012; Murthy et al. 2014).”
Discovery and resupply of pharmacologically active plant-derived natural products: A review (2015, background): “These are the inoculum density, temperature, light quality and intensity, pH of the nutrient medium, and agitation and aeration of the culture batch (Kanokwaree and Doran, 1997; Murthy et al., 2014).”
Elicitor induced stevioside production, in vitro shoot growth, and biomass accumulation in micropropagated Stevia rebaudiana (2016, background): “Elicitors are compounds triggering the formation of secondary metabolites, which have been widely used for the overproduction of secondary metabolites in in vitro plant cultures (Dörnenburg and Knorr 1995; Ramakrishna and Ravishankar 2011; Murthy et al. 2014).”