Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy
By R. Barretto, T. Ko, Juergen C. Jung, Tammy J. Wang, G. Capps, A. Waters, Yaniv Ziv, A. Attardo, L. Recht, Mark J. Schnitzer. 2011
246 citations recorded by the source corpus.
The combination of intravital microscopy and animal models of disease has propelled studies of disease mechanisms and treatments. However, many disorders afflict tissues inaccessible to light microscopy in live subjects. Here we introduce cellular-level time-lapse imaging deep within the live mammalian brain by one- and two-photon fluorescence microendoscopy over multiple weeks. Bilateral imaging sites allowed longitudinal comparisons within individual subjects, including of normal and diseased tissues. Using this approach, we tracked CA1 hippocampal pyramidal neuron dendrites in adult mice, revealing these dendrites' extreme stability and rare examples of their structural alterations. To illustrate disease studies, we tracked deep lying gliomas by observing tumor growth, visualizing three-dimensional vasculature structure and determining microcirculatory speeds. Average erythrocyte speeds in gliomas declined markedly as the disease advanced, notwithstanding significant increases in capillary diameters. Time-lapse microendoscopy will be applicable to studies of numerous disorders, including neurovascular, neurological, cancerous and trauma-induced conditions.
Subfields: Biomedical Engineering, Biophysics, Neurology
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Neurology
Citations in the corpus
- Adult Neurogenesis in the Mammalian Brain: Significant Answers and Significant Questions (2011, methodology): “, 2008) and Split-Cre (Beckervordersandforth et al., 2010), and advances in the technology of next-generation sequencing and metabolomics will greatly facilitate the effort.”
- Tracking the Same Neurons across Multiple Days in Ca2+ Imaging Data (2017, methodology): “We combined miniaturized head-mounted microscopes (Ghosh et al., 2011) and microendoscopic probes (Barretto et al., 2011) to chronically image Ca2+ dynamics in GCaMP6expressing neurons in the hippocampal CA1 and in the prefrontal cortex of freely behaving mice.”