Functional Analysis of an Arabidopsis Transcription Factor, DREB2A, Involved in Drought-Responsive Gene Expression[W][OA]

By Y. Sakuma, K. Maruyama, Yuriko Osakabe, Feng Qin, Motoaki Seki, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki. The Plant Cell Online, 2006

1,026 citations recorded by the source corpus.

Transcription factors DREB1A/CBF3 and DREB2A specifically interact with cis-acting dehydration-responsive element/C-repeat (DRE/CRT) involved in cold and drought stress–responsive gene expression in Arabidopsis thaliana. Intact DREB2A expression does not activate downstream genes under normal growth conditions, suggesting that DREB2A requires posttranslational modification for activation, but the activation mechanism has not been clarified. DREB2A domain analysis using Arabidopsis protoplasts identified a transcriptional activation domain between residues 254 and 335, and deletion of a region between residues 136 and 165 transforms DREB2A to a constitutive active form. Overexpression of constitutive active DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. Microarray and RNA gel blot analyses revealed that DREB2A regulates expression of many water stress–inducible genes. However, some genes downstream of DREB2A are not downstream of DREB1A, which also recognizes DRE/CRT but functions in cold stress–responsive gene expression. Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A but only a weak signal when fused to full-length DREB2A. The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/42b2bbc007f40089bd98af9ac558c237e6b59b08

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Citations in the corpus

Transcription factors in plants and ABA dependent and independent abiotic stress signalling (2010, background): “Microarray analysis of AtDREB2A transgenic plants have shown overexpression of the 21 genes, of these 14 genes were upregulated by drought, 9 of which encode LEA class proteins, which are thought to protect macromolecules, such as enzymes and lipids from dehydration (Sakuma et al. 2006a).”
Effects of abiotic stress on plants: a systems biology perspective (2011, background): “DREB1/ CBFs function in cold-responsive gene expression [48,49], whereas DREB2s are involved in dehydrationresponsive and heat-responsive gene expression [50].”
Response of plants to water stress (2014, background): “…gene transcription have also been identified, such as MYB, MYC, DREB/CBF (drought-responsive cis-element binding protein/C-repeat-binding factor), ABF/AREB, NAC, and WRKY TFs (Stockinger et al., 1997; Sakuma et al., 2006; Tran et al., 2007b; Nakashima et al., 2009; Ishida et al., 2012; Figure 2).”
Transgenic Plants for Abiotic Stress Resistance (2010, background): “DREB2A overexpression in Arabidopsis confers significant drought tolerance in transgenic plants (Sakuma et al. 2006a, b).”
Salt-tolerant genes from halophytes are potential key players of salt tolerance in glycophytes (2016, background): “In another study, the middle region of DREB2A functions as a negative regulatory domain (NRD), and the removal of this region resulted in the constitutive activation of the DREB transcription factor and provided tolerance under various abiotic stresses (Sakuma et al., 2006).”
Antibiotic resistance plasmids in wastewater treatment plants and their possible dissemination into the environment (2010, background): “in enhanced thermotolerance (Sakuma et al., 2006; Qin et al., 2007).”
Signal transduction during cold, salt, and drought stresses in plants (2011, background): “Thus, these results suggest that stability of the DREB2A protein is important for its activation, and the activated DREB2A regulates drought stress–responsive gene expression, which enhances drought stress tolerance in plants [85].”
Genetic correlation and path analysis of transpiration efficiency for wheat flag leaves (2008, neutral): “As a quantitative trait under multi-gene control, water-use efficiency is associated with many underlying and inter-related traits and is affected by environmental factors and redox (reactive oxygen species) (Shao et al., 2005f; Torres et al., 2006; Sakuma et al., 2006; Asada, 2006).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “, 2018b), whereas DREB2s function in drought response (Sakuma et al., 2006a).”